Circulating factors that modify lung cell DNA synthesis following exposure to inhaled oxidants. I. Effect of serum and lavage on lung fibroblasts following exposure of adult rats to 1 ppm ozone

Journal Article · Sun Jan 01 04:00:00 EST 1989 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398909531294· OSTI ID:5764359

Tanswell, A K; Fraher, L J; Grose, E C

Adult rats were exposed to 1 ppm (1.96 mg/m/sup 3/) ozone or air for 2 wk. Animals were sacrificed at 3, 5, 7, or 14 d after the onset of exposure, and samples of plasma and lung lavage were obtained. Heat-inactivated plasma, from animals exposed to ozone for 7 or 14 d, significantly increased DNA synthesis by lung fibroblasts compared with plasma from air-exposed animals. Fractionation of plasma and lavage samples indicated that the factor responsible had an isoelectric point of 6.45-6.75 and a molecular weight of 32 +/- 2 kDa. This factor has a dose-dependent effect on lung fibroblast DNA synthesis in culture, but no significant effect on cultured pneumocyte DNA synthesis. The factor is detectable within 72 h of exposure, and may hold some promise as a marker of early oxidant lung injury.

• Research Organization: Univ. of Western Ontario, London (Canada)
• OSTI ID: 5764359
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 27:2; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOASSAY
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY
BODY FLUIDS
CELL CULTURES
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
CULTURE MEDIA
DNA
DNA REPLICATION
ENVIRONMENTAL EXPOSURE
EXPOSURE CHAMBERS
FIBROBLASTS
FRACTIONATION
GROWTH FACTORS
LAVAGE
LUNGS
MAMMALS
MATERIALS
MITOGENS
MOLECULAR WEIGHT
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OZONE
PROTEINS
RATS
RESPIRATORY SYSTEM
RODENTS
SENSITIVITY
SEPARATION PROCESSES
SOMATIC CELLS
TOXICITY
VERTEBRATES