Circulating factors that modify lung-cell DNA synthesis following exposure to inhaled oxidants. 1. Effect of serum and lavage on lung fibroblasts following exposure of adult rats to 1 ppm ozone

Technical Report · Sun Jan 01 04:00:00 EST 1989

OSTI ID:6967749

Tanswell, A K; Fraher, L J; Grose, E C

Adult rats were exposed to 1 ppm (1.96 mg/cu m) ozone or air for 2 weeks. Animals were sacrificed at 3, 5, 7, or 14 days after the onset of exposure and samples of plasma and lung lavage obtained. Heat inactivated plasma, from animals exposed to ozone for 7 or 14 days, significantly increased DNA synthesis by lung fibroblasts compared with plasma from air exposed animals. Fractionation of plasma and lavage samples indicated that the factor responsible had an isoelectric point of 6.45-6.75, and a molecular weight of 32 +/- 2 Kd. This factor has a dose-dependent effect on lung fibroblasts in culture, but no significant effect on cultured pneumocyte DNA synthesis. The factor is detectable within 72 hours of exposure, and may hold some promise as a marker of early oxidant lung injury.

• Research Organization: Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
• OSTI ID: 6967749
• Report Number(s): PB-90-146143/XAB; EPA--600/J-89/216
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOASSAY
BIOLOGICAL MARKERS
BODY
CONNECTIVE TISSUE CELLS
DNA REPLICATION
FIBROBLASTS
INHALATION
INTAKE
LAVAGE
LUNGS
NUCLEIC ACID REPLICATION
ORGANS
OZONE
PATHOLOGICAL CHANGES
RESPIRATION
RESPIRATORY SYSTEM
SOMATIC CELLS
TOXICITY