Investigation of prostaglandin levels in human milk after high performance liquid chromatography purification

Conference · Tue Mar 04 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7189320

Wu-Wang, C Y; Neu, J

This study was conducted to investigate five prostaglandins (PGs), i.e. PGE/sub 2/, PGF/sub 2..cap alpha../, 13-14-dihydro-15-keto-PGF/sub 2..cap alpha../ (DHKF/sub 2..cap alpha../), thromboxane B/sub 2/ (TXB/sub 2/) and 6-keto-PGF/sub 1..cap alpha../), measured by (RIA) after C/sub 18/ Sep-Pak extraction and reverse phase high performance liquid chromatography (HPLC). Two trials were performed. In each trial, 3-5 mature human milk samples were pooled, acidified and extracted for PGs. The separation of PGs by HPLC was achieved by using an isocratic solvent system of acetonitrile/water (pH 3.0) (32/68, V/V). The PG levels from the two trials were determined and averaged after monitoring the recoveries. The results indicate that PGE/sub 2/ and DHKF/sub 2..cap alpha../ are the two major PGs found in extracted human milk. However, after HPLC purification, no predominant PG is found and the levels of all the five PGs are much lower compared to the extracted sample. Since the immunoreactive material was also detected in HPLC fractions not within the PG peak, low levels of PG found in human milk after HPLC is likely due to the purification step removing the bulk of nonspecific immunoreactive substances present in the sample.

• Research Organization: Univ. of Florida, Gainesville
• OSTI ID: 7189320
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL MATERIALS
BODY FLUIDS
CHROMATOGRAPHY
FOOD
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MATERIALS
MILK
PRIMATES
PROSTAGLANDINS
PURIFICATION
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES