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Very low levels of 6-keto-prostaglandin F /sub 1//sub. cap alpha. / in human plasma

Journal Article · · J. Lab. Clin. Med.; (United States)
OSTI ID:5505028
;

Two stable derivatives of PGI/sub 2/, its nonenzymatic hydrolysis product (6-keto-PGF/sub 1//sub ..cap alpha../) and an enzymatic metabolite (6, 15-diketo-PGF/sub 1//sub ..cap alpha../) were determined in human plasma and urine. These compounds were measured by RIA after separation on rp-HPLC. Previous purification of the samples on rp=HPLC markedly enhanced the specificity of the RIA determinations of those compounds in plasma and urine. The PGI/sub 2/ derivative 6-keto-PGF/sub 1//sub ..cap alpha../ was detected in both plasma (4.7 +/- 3.2 pg/ml, mean +/- S.D., n = 34) and urine (166 +/- 61 pg/ml, n = 9). No gender differences of the plasma or urinary levels of 6-keto-PGF/sub 1//sub ..cap alpha../ were found. The PGI/sub 2/ metabolite 6, 15-diketo-PGF/sub 1//sub ..cap alpha../ was not measurable in plasma or urine (<1 and <10 pg/ml, respectively, n = 4). Thus this compound may not be a major endogenous metabolite of PGI/sub 2/ in man. When (/sup 3/H)PGI/sub 2/ was added to citrated blood immediately after venipuncture, it was recovered entirely as (/sup 3/H)6-keto-PGF/sub 1//sub ..cap alpha../ after rp-HPLC. Therefore any circulating PGI/sub 2/ would be measured as 6-keto-PGF/sub 1//sub ..cap alpha../ by our method. The results obtained suggest that PGI/sub 2/ could be present in human venous blood under physiological conditions, but only in very low concentrations.

Research Organization:
Institut Pasteur, Paris France
OSTI ID:
5505028
Journal Information:
J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 99:3; ISSN JLCMA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BLOOD PLASMA
BODY FLUIDS
CHROMATOGRAPHY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MATERIALS
METABOLITES
PATIENTS
PROSTAGLANDINS
RADIOASSAY
RADIOIMMUNOASSAY
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URINE
WASTES