Toxicity of hexavalent chromium to the alveolar macrophage in vivo and in vitro. [Rats]

Journal Article · Wed Feb 01 04:00:00 EST 1984 · Environ. Res.; (United States)

OSTI ID:7188735

Galvin, J B; Oberg, S G

In vivo and in vitro systems were used to evaluate hexavalent chromium toxicity to alveolar macrophages. Rat alveolar macrophages were exposed to 2 ..mu..g calcium chromate (CaCrO/sub 4/, insoluble) or 2 ..mu..g chromium trioxide (CrO/sub 3/, soluble) in live animals, in vivo, and in tissue culture, in vitro, collected by lavage from the lung. Chemiluminescence and oxygen consumption were measured as indicators of toxicity. Trypan blue dye exclusion was used to determine macrophage viability. In vivo exposure of the macrophage to either chromium compound showed no toxic effects at a 2-..mu..g dose. Macrophages exposed in tissue culture, however, had values significantly different from controls. The untreated controls for both exposure methods were compared to evaluate differences resulting from methods alone.

• Research Organization: Utah State Univ., Logan
• OSTI ID: 7188735
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 33:1; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BODY
CHEMILUMINESCENCE
CHROMIUM COMPOUNDS
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
FUNCTIONS
IN VITRO
IN VIVO
LUMINESCENCE
LUNGS
MACROPHAGES
MAMMALS
MEASURING METHODS
ORGANS
PHAGOCYTES
RATS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES