Relative effects of asbestos and wollastonite on alveolar macrophages

Journal Article · Sun Jan 01 04:00:00 EST 1984 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398409530601· OSTI ID:5885221

Pailes, W H; Judy, D J; Resnick, H; Castranova, V

Rabbit alveolar macrophages were exposed in culture to chrysotile asbestos, wollastonite, or latex, and the effects on various biochemical and physiological parameters related to cellular viability and fibrogenicity were determined. Exposure of alveolar macrophages to asbestos, wollastonite, or latex for 3 d has no effect on oxygen consumption or cellular volume. However, treatment of alveolar macrophages with as little as 25 ..mu..g asbestos/ml for 1 d increases lysosomal enzyme release and decreases membrane integrity, i.e., decreases trypan blue exclusion and increases leakage of cytosolic enzymes. In contrast, exposure of alveolar macrophages to wollastonite or latex at 250 ..mu..g/ml does not induce lysosomal enzyme release or alter membrane integrity even after 3 d of exposure in culture. These data suggest that chrysotile asbestos damages rabbit alveolar macrophages, while wollastonite, a potential substitute for asbestos, is far less cyctotoxic. 35 references, 8 figures.

• Research Organization: National Inst. for Occupational Safety and Health, Morgantown, WV
• OSTI ID: 5885221
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 14:4; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ASBESTOS
BODY
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
DATA
ELASTOMERS
ENZYME ACTIVITY
EXPERIMENTAL DATA
INFORMATION
INJURIES
LATEX
LUNGS
MACROPHAGES
MAMMALS
MEMBRANES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC POLYMERS
ORGANS
PHAGOCYTES
POLYMERS
RABBITS
RESPIRATORY SYSTEM
RUBBERS
SOMATIC CELLS
STIMULATION
TOXICITY
VERTEBRATES
VIABILITY