Cytotoxicity of ammonium metavanadate to cultured bovine alveolar macrophages

Journal Article · Sat May 01 04:00:00 EDT 1982 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398209530220· OSTI ID:5019086

Wei, C; Misra, H P

Cytotoxicity of ammonium metavanadate to cultured bovine pulmonary alveolar macrophages was measured by cell viability, inhibition of phagocytosis, and reduction of superoxide-dependent chemiluminescence. The degree of toxicity was dependent on the levels of vanadium, the temperature, and the time of exposure. Thus macrophages exposed to vanadium at 0.01 and 0.1 ..mu..g/ml did not exhibit cytotoxic effects even with up to 24 h of exposure, as measured by cell viability and phagocytic index. Vanadium at 0.5 ..mu..g/ml, however, reduced cell viability to 24% and the phagocytic index to 2% of the control within 8 h of exposure. Exposure to NH/sub 4/VO/sub 3/ (up to 1 ..mu..g vanadium/ml) for short periods of time stimulated phagocytic activity. Vanadium toxicity was also demonstrated in suspension culture at 37/sup 0/C by chemiluminescence assay. This assay seems to be more sensitive than the conventional viability and phagocytic index tests. Thus, the peak light production by macrophages during zymosan phagoctyosis was reduced to 93, 59, and 63% by vanadium at 0.1 ..mu..g/ml exposing for 2, 4, and 8 h, respectively, and to 71, 27, and 24% by vanadium at 1.0 ..mu..g/ml for the same time periods. The phagoctyic activity of macrophages as measured by chemiluminescence response was not significantly altered by exposure to either 0.1 or 1.0 ..mu..g vanadium/ml measured during the first 24 h of culture at 4/sup 0/C.

• Research Organization: Univ. of California, Davis
• OSTI ID: 5019086
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 9:5-6; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOL WASTES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
ASHES
BIOLOGICAL EFFECTS
BODY
CATTLE
CELL CULTURES
CHEMILUMINESCENCE
CONNECTIVE TISSUE CELLS
COWS
DOMESTIC ANIMALS
DOSE-RESPONSE RELATIONSHIPS
FLY ASH
LUMINESCENCE
LUNGS
MACROPHAGES
MAMMALS
ORGANS
PHAGOCYTES
PHAGOCYTOSIS
RESIDUES
RESPIRATORY SYSTEM
RUMINANTS
SOMATIC CELLS
TRANSITION ELEMENT COMPOUNDS
VANADIUM COMPOUNDS
VERTEBRATES
VIABILITY
WASTES