Quantitative assessment of rabbit alveolar macrophage function by chemiluminescence

Journal Article · Thu Aug 01 04:00:00 EDT 1985 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/0013-9351(85)90127-6· OSTI ID:5093556

Brennan, P C; Kirchner, F R

Rabbit alveolar macrophages (RAM) were cultured for 24 hr with concentrations ranging from 3 to 12 ..mu..g/ml of vanadium oxide (V/sub 2/O/sub 5/), a known cytotoxic agent, or with high-molecular-weight organic by-products from coal gasification processes. After culture the cells were harvested and tested for functional capacity using three types of indicators: (1) luminol-amplified chemiluminescence (CL), which quantitatively detects photon emission due to respiratory burst activity measured in a newly designed instrument with standardized reagents; (2) the reduction of nitro blue tetrazolium-saturated polyacrylamide beads, a semiquantitative measure of respiratory burst activity; and (3) phagocytic efficiency, defined as percentage of cells incorporating immunoglobulin-coated polyacrylamide beads. Chemiluminescence declined linearly with increasing concentrations of V/sub 2/O/sub 5/ over the dose range tested. Dye reduction and phagocytic efficiency similarly decreased with increasing V/sub 2/O/sub 5/ concentration, but were less sensitive indicators of functional impairment than CL as measured by the amount required to reduce the response to 50% of untreated cells. The effect of coal gasification condensates on RAM function varied, but in general these test also indicated that the CL response was the most sensitive indicator.

• Research Organization: Packard Instrument Co., Downers Grove, IL
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 5093556
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 37:2; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL FUNCTIONS
CELL CULTURES
CHALCOGENIDES
CHEMILUMINESCENCE
COAL GASIFICATION
COMBUSTION PRODUCTS
CONNECTIVE TISSUE CELLS
DATA
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
FUNCTIONS
GASIFICATION
INFORMATION
LUMINESCENCE
MACROPHAGES
MAMMALS
MEASURING METHODS
NUMERICAL DATA
OXIDES
OXYGEN COMPOUNDS
PHAGOCYTES
PHAGOCYTOSIS
RABBITS
SOMATIC CELLS
THERMOCHEMICAL PROCESSES
TOXICITY
TRANSITION ELEMENT COMPOUNDS
VANADIUM COMPOUNDS
VANADIUM OXIDES
VERTEBRATES