Stimulation of alveolar macrophages by mineral dusts in vitro: luminol-dependent chemiluminescence study

Journal Article · Sun Feb 01 04:00:00 EST 1987 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/S0013-9351(87)80026-9· OSTI ID:6785758

Vilim, V; Wilhelm, J; Brzak, P; Hurych, J

Luminol-dependent chemiluminescence (CL) of normal (nonactivated) rabbit alveolar macrophages (AMs) was measured in suspension upon stimulation by various size fractions of one quartz dust sample or by various mineral dusts (quartz, corundum, anatas, and chrysotile asbestos as an example of fibrous dust). The CL-triggering capacity of the tested dusts was inhibited by their preincubation with autologous serum. The intensity of luminol-dependent CL induced by particulate dusts upon their action on AMs depended on the kind of dust, on the dust particle sizes, and on the ratio of the number of particles to the number of cells in a given suspension. The cytotoxicity and/or fibrogenicity of the dust and its capacity to trigger the luminol-dependent CL of nonadherent AMs were not directly correlated.

• Research Organization: Institute of Hygiene and Epidemiology, Prague, Czechoslovakia
• OSTI ID: 6785758
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 42:1; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALUMINIUM COMPOUNDS
ALUMINIUM OXIDES
ANIMAL CELLS
ANIMALS
ASBESTOS
BODY
CHALCOGENIDES
CHEMILUMINESCENCE
CONNECTIVE TISSUE CELLS
CORUNDUM
DUSTS
FIBERS
LUMINESCENCE
LUNGS
MACROPHAGES
MAMMALS
MINERALS
ORGANS
OXIDE MINERALS
OXIDES
OXYGEN COMPOUNDS
PARTICLE SIZE
PARTICLES
PARTICULATES
PHAGOCYTES
QUARTZ
RABBITS
RESPIRATORY SYSTEM
SILICON COMPOUNDS
SILICON OXIDES
SIZE
SOMATIC CELLS
STIMULATION
TOXICITY
VERTEBRATES