Estrogen binding, receptor mRNA, and biologic response in osteoblast-like osteosarcoma cells

Journal Article · Fri Jul 01 04:00:00 EDT 1988 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.3164526· OSTI ID:7188183

Komm, B S; Terpening, C M; Benz, D J; Graeme, K A; Gallegos, A; Korc, M; Greene, G L; O'Malley, B W; Haussler, M R

High specific activity estradiol labeled with iodine-125 was used to detect approximately 200 saturable, high-affinity (dissociation constant approximately equal to 1.0 nM) nuclear binding sites in rat (ROS 17/2.8) and human (HOS TE85) clonal osteoblast-like osteosarcoma cells. Of the steroids tested, only testosterone exhibited significant cross-reactivity with estrogen binding. RNA blot analysis with a complementary DNA probe to the human estrogen receptor revealed putative receptor transcripts of 6 to 6.2 kilobases in both rat and human osteosarcoma cells. Type I procollagen and transforming growth factor-beta messenger RNA levels were enhanced in cultured human osteoblast-like cells treated with 1 nM estradiol. Thus, estrogen can act directly on osteoblasts by a receptor-mediated mechanism and thereby modulate the extracellular matrix and other proteins involved in the maintenance of skeletal mineralization and remodeling.

• Research Organization: Univ. of Arizona College of Medicine, Tucson (USA)
• OSTI ID: 7188183
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 241:4861; ISSN SCIEA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
ELECTRON CAPTURE RADIOISOTOPES
ESTRADIOL
ESTRANES
ESTROGENS
GROWTH
GROWTH FACTORS
HORMONES
HYBRIDIZATION
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MAN
MEMBRANE PROTEINS
MESSENGER-RNA
MITOGENS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
OSTEOSARCOMAS
PEPTIDES
PRIMATES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RNA
RODENTS
SARCOMAS
SKELETAL DISEASES
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
TRANSCRIPTION
TUMOR CELLS
VERTEBRATES