Identification of androgen receptors in normal human osteoblast-like cells

Journal Article · Tue Jan 31 23:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.3.854· OSTI ID:5031113

Colvard, D S; Eriksen, E F; Keeting, P E; Riggs, B L; Spelsberg, T C ^[1]; Wilson, E M; Lubahn, D B; French, F S ^[2]

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1. Mayo Clinic and Mayo Foundation, Rochester, MN (USA)
2. Univ. of North Carolina, Chapel Hill (USA)

The sex steroids, androgens and estrogens, are major regulators of bone metabolism. However, whether these hormones act on bone cells through direct or indirect mechanisms has remained unclear. A nuclear binding assay recently used to demonstrate estrogen receptors in bone was used to identify specific nuclear binding of a tritiated synthetic androgen, ({sup 3}H)R1881 (methyltrienolone), in 21 of 25 (84%) human osteoblast-like cell strains and a concentration of bound steroid receptors of 821 {plus minus} 140 molecules per cell nucleus. Binding was saturable and steroid-specific. Androgen receptor gene expression in osteoblasts was confirmed by RNA blot analysis. Relative concentrations of androgen and estrogen receptors were compared by measuring specific nuclear estrogen binding. Nuclear binding of ({sup 3}H)estradiol was observed in 27 of 30 (90%) cell strains; the concentration of bound estradiol receptor was 1537 {plus minus} 221 molecules per cell nucleus. The concentrations of nuclear binding sites were similar in males and females for both ({sup 3}H)R1881 and ({sup 3}H)estradiol. The authors conclude that both androgens and estrogens act directly on human bone cells through their respective receptor-mediated mechanisms.

• OSTI ID: 5031113
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:3; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANDROGENS
ANDROSTANES
ANIMAL CELLS
ANIMAL TISSUES
BODY
BONE CELLS
BONE TISSUES
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
ESTRADIOL
ESTRANES
ESTROGENS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PHYSIOLOGY
PROTEINS
RADIORECEPTOR ASSAY
RECEPTORS
SOMATIC CELLS
STEROID HORMONES
STEROIDS
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS