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Title: Kernel abortion in maize. II. Distribution of /sup 14/C among kernel carboydrates

Abstract

This study was designed to compare the uptake and distribution of /sup 14/C among fructose, glucose, sucrose, and starch in the cob, pedicel, and endosperm tissues of maize (Zea mays L.) kernels induced to abort by high temperature with those that develop normally. Kernels cultured in vitro at 309 and 35/sup 0/C were transferred to (/sup 14/C)sucrose media 10 days after pollination. Kernels cultured at 35/sup 0/C aborted prior to the onset of linear dry matter accumulation. Significant uptake into the cob, pedicel, and endosperm of radioactivity associated with the soluble and starch fractions of the tissues was detected after 24 hours in culture on atlageled media. After 8 days in culture on (/sup 14/C)sucrose media, 48 and 40% of the radioactivity associated with the cob carbohydrates was found in the reducing sugars at 30 and 35/sup 0/C, respectively. Of the total carbohydrates, a higher percentage of label was associated with sucrose and lower percentage with fructose and glucose in pedicel tissue of kernels cultured at 35/sup 0/C compared to kernels cultured at 30/sup 0/C. These results indicate that sucrose was not cleaved to fructose and glucose as rapidly during the unloading process in the pedicel of kernels induced tomore » abort by high temperature. Kernels cultured at 35/sup 0/C had a much lower proportion of label associated with endosperm starch (29%) than did kernels cultured at 30/sup 0/C (89%). Kernels cultured at 35/sup 0/C had a correspondingly higher proportion of /sup 14/C in endosperm fructose, glucose, and sucrose.« less

Authors:
;
Publication Date:
Research Org.:
Univ. of Minnesota, St. Paul
OSTI Identifier:
7188081
Resource Type:
Journal Article
Resource Relation:
Journal Name: Plant Physiol.; (United States); Journal Volume: 81:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CARBON 14 COMPOUNDS; TISSUE DISTRIBUTION; UPTAKE; HYPERTHERMIA; BIOLOGICAL EFFECTS; STARCH; BIOSYNTHESIS; CARBOHYDRATES; INHIBITION; KERNELS; MAIZE; SACCHARIDES; TEMPERATURE EFFECTS; BODY TEMPERATURE; CEREALS; DISTRIBUTION; GRASS; LABELLED COMPOUNDS; ORGANIC COMPOUNDS; PLANTS; POLYSACCHARIDES; REAGENTS; SYNTHESIS 551001* -- Physiological Systems-- Tracer Techniques

Citation Formats

Hanft, J.M., and Jones, R.J.. Kernel abortion in maize. II. Distribution of /sup 14/C among kernel carboydrates. United States: N. p., 1986. Web.
Hanft, J.M., & Jones, R.J.. Kernel abortion in maize. II. Distribution of /sup 14/C among kernel carboydrates. United States.
Hanft, J.M., and Jones, R.J.. 1986. "Kernel abortion in maize. II. Distribution of /sup 14/C among kernel carboydrates". United States. doi:.
@article{osti_7188081,
title = {Kernel abortion in maize. II. Distribution of /sup 14/C among kernel carboydrates},
author = {Hanft, J.M. and Jones, R.J.},
abstractNote = {This study was designed to compare the uptake and distribution of /sup 14/C among fructose, glucose, sucrose, and starch in the cob, pedicel, and endosperm tissues of maize (Zea mays L.) kernels induced to abort by high temperature with those that develop normally. Kernels cultured in vitro at 309 and 35/sup 0/C were transferred to (/sup 14/C)sucrose media 10 days after pollination. Kernels cultured at 35/sup 0/C aborted prior to the onset of linear dry matter accumulation. Significant uptake into the cob, pedicel, and endosperm of radioactivity associated with the soluble and starch fractions of the tissues was detected after 24 hours in culture on atlageled media. After 8 days in culture on (/sup 14/C)sucrose media, 48 and 40% of the radioactivity associated with the cob carbohydrates was found in the reducing sugars at 30 and 35/sup 0/C, respectively. Of the total carbohydrates, a higher percentage of label was associated with sucrose and lower percentage with fructose and glucose in pedicel tissue of kernels cultured at 35/sup 0/C compared to kernels cultured at 30/sup 0/C. These results indicate that sucrose was not cleaved to fructose and glucose as rapidly during the unloading process in the pedicel of kernels induced to abort by high temperature. Kernels cultured at 35/sup 0/C had a much lower proportion of label associated with endosperm starch (29%) than did kernels cultured at 30/sup 0/C (89%). Kernels cultured at 35/sup 0/C had a correspondingly higher proportion of /sup 14/C in endosperm fructose, glucose, and sucrose.},
doi = {},
journal = {Plant Physiol.; (United States)},
number = ,
volume = 81:2,
place = {United States},
year = 1986,
month = 6
}