Transport and metabolism of a sucrose analog (1'-fluorosucrose) into Zea mays L. Endosperm without invertase hydrolysis

Journal Article · Tue Dec 01 04:00:00 EST 1987 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.85.4.902· OSTI ID:5600485

Schmalstig, J G; Hitz, W D

1'-fluorosucrose (FS), a sucrose analog resistant to hydrolysis by invertase, was transported from husk leaves into maize (Zea mays L.) kernels with the same magnitude and kinetics as sucrose. /sup 14/C-Label from (/sup 14/C)FS and (/sup 14/C)sucrose in separate experiments was distributed similarly between the pedicel, endosperm, and embryo with time. FS passed through maternal tissue and was adsorbed intact into the endosperm where it was metabolized and used in synthesis of sucrose and methanol-chloroform-water insolubles. Accumulation of (/sup 14/C)sucrose from supplied (/sup 14/C)glucosyl-FS indicated that the glucose moiety from the breakdown of sucrose (here FS), which normally occurs in the process of starch synthesis in maize endosperm, was available to the pool of substrates for resynthesis of sucrose. Uptake of FS into maize endosperm without hydrolysis suggest that despite the presence of invertase in maternal tissues and the hydrolysis of a large percentage of sucrose unloaded form the phloem, hexoses are not specifically needed for uptake into maize endosperm.

• Research Organization: E. I. DuPont de Nemours and Company, Wilmington, DE
• OSTI ID: 5600485
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 85:4; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
CARBOHYDRATES
CARBON 14 COMPOUNDS
CEREALS
CHEMICAL REACTIONS
DECOMPOSITION
DISACCHARIDES
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
GRASS
HYDROLYSIS
ISOMERASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYSIS
MAIZE
MEMBRANE TRANSPORT
METABOLISM
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
SACCHAROSE
SOLVOLYSIS
TRACER TECHNIQUES