Effect of pretreatment with pentobarbital on the extent of (/sup 14/C) incorporation from (U-/sup 14/C)glucose into various rat brain glycolytic intermediates: relevance to regulation at hexokinase and phosphofructokinase

Journal Article · Fri Apr 01 04:00:00 EST 1988 · Neurochem. Res.; (United States)

DOI:https://doi.org/10.1007/BF00972488· OSTI ID:7187831

Miller, L P; Mayer, S; Braun, L D; Geiger, P; Oldendorf, W H

In the present investigation we monitored the incorporation of (14C) from (U-14C)glucose into various rat brain glycolytic intermediates of conscious and pentobarbital-anesthetized animals. Labeled glucose was delivered to brain by single bolus intracarotid injection and brain tissue was subsequently prepared at 15, 30, and 45 sec by freeze-blowing. Glycolytic intermediates were then separated by column chromatography. Our results showed a gradual decrease with time of 14C-labeled glucose which gave a calculated rate for glucose metabolism of 0.86 mumol/min/g and 0.56 mumol/min/g in conscious and anesthetized animals, respectively. Compared to the results obtained using conscious animals the administration of pentobarbital not only resulted in a significant attenuation of the rate of glucose metabolism but also caused a similar reduction in the amount of 14C incorporated into several glycolytic intermediates. These intermediates included: glucose 6-phosphate, fructose 6-phosphate, fructose 1,6 diphosphate, dihydroxyacetone phosphate and post glycolytic compounds. In addition, pretreatment with pentobarbital resulted in a 75% increase in the endogenous concentration of glucose, 10% increase in glucose 6-phosphate, no change in fructose 6-phosphate and 42% decrease in lactate compared to levels in brains obtained from conscious animals. These results are discussed in relation to control of glycolysis through coupled regulation at hexokinase-phosphofructokinase.

• Research Organization: West Los Angeles VA Medical Center, CA (USA)
• OSTI ID: 7187831
• Journal Information: Neurochem. Res.; (United States), Journal Name: Neurochem. Res.; (United States) Vol. 13:4; ISSN NERED
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANESTHETICS
ANIMALS
AZINES
BARBITURATES
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CARBOHYDRATES
CARBON 14 COMPOUNDS
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHROMATOGRAPHY
DRUGS
ENZYMES
GLUCOSE
GLYCOLYSIS
HETEROCYCLIC COMPOUNDS
HEXOKINASE
HEXOSES
HOMEOSTASIS
HYPNOTICS AND SEDATIVES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
METABOLISM
MONOSACCHARIDES
NEMBUTAL
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PYRIMIDINES
RATS
REACTION KINETICS
RODENTS
SACCHARIDES
SEPARATION PROCESSES
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES