Alterations in glucose kinetics induced by pentobarbital anesthesia

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6926898

Lang, C H; Bagby, G J; Spitzer, J J

Pentobarbital is a common anesthetic agent used in animal research that is known to alter sympathetic function and may also affect carbohydrate metabolism. The in vivo effects of iv pentobarbital on glucose homeostasis were studied in chronically catheterized fasted rats. Whole body glucose kinetics, assessed by the constant iv infusion of (6-/sup 3/H)- and (U-/sup 14/C)-glucose, were determined in all rats in the conscious state. Thereafter, glucose metabolism was followed over the next 4 hr in 3 subgroups of rats; conscious, anesthetized with body temperature maintained, and anesthetized with body temperature not maintained. Hypothermia (a 5/sup 0/C decrease) developed spontaneously in anesthetized rats kept at ambient temperature (22/sup 0/C). No differences were seen in MABP and heart rate between conscious and normothermic anesthetized rats; however, hypothermic anesthetized rats showed a decrease in MABP (20%) and heart rate (35%). Likewise, plasma glucose and lactate concentrations, the rate of glucose appearance (Ra), recycling and metabolic clearance (MCR) did not differ between conscious and normothermic anesthetized animals. In contrast, hypothermic anesthetized rats showed a 50% reduction in plasma lactate, a 40% drop in glucose Ra, and a 30-40% decrease in glucose recycling and MCR. Thus, pentobarbital does not appear to alter in vivo glucose kinetics, compared to unanesthetized controls, provided that body temperature is maintained.

• Research Organization: Louisiana State Univ. Medical Center, New Orleans
• OSTI ID: 6926898
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANESTHETICS
ANIMALS
AZINES
BARBITURATES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY TEMPERATURE
CARBOHYDRATES
CARBON 14 COMPOUNDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CLEARANCE
DRUGS
GLUCOSE
HETEROCYCLIC COMPOUNDS
HEXOSES
HYPNOTICS AND SEDATIVES
HYPOTHERMIA
KINETICS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
MONOSACCHARIDES
NEMBUTAL
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PYRIMIDINES
RATS
REACTION KINETICS
RODENTS
SACCHARIDES
TRITIUM COMPOUNDS
VERTEBRATES