Preferential enhancement of reverse dermal Arthus reaction by polyelectrolytes: in vivo and in vitro evidence for mediation by oxygen-derived radicals and their metabolites

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7186451

Ginsberg, I; Warren, J S; Johnson, K J; Ward, P A

The ability of cationic and anionic polyelectrolytes (polyhistidine, histone, poly-arginine and polyanetholsulfonate) to modulate an acute immune complex (IgG-BSA) mediated inflammatory response was studied. Tissue injury elicited in rats by the reverse dermal Arthus reaction was increased 20-60% by complexing the antibody and polyelectrolye prior to intradermal injection. Intravenous administration of polyethylene glycol-coupled (PEG) superoxide dismutase (4125 U) produced a 30-70% suppression of this tissue injury. PEG-catalase (2000 U) had no suppressive effect. Concomitant in vitro functional studies (enzyme release, O/sub 2//sup -/ and H/sub 2/O/sub 2/ generation and chemiluminescence) of rat neutrophils stimulated with preformed immune complexes modified with polyelectrolytes demonstrated 2-fold increase in O/sub 2//sup -/ generation, modest increases in H/sub 2/O/sub 2/ generation and large increases in chemiluminescence. There was no change in enzyme (..beta..-glucuronidase) secretion. The polyelectrolytes employed in this study did not alter the capacity of preformed IgG-BSA complexes to fix complement. These studies suggest that immune complexes modified with either cationic or anionic polyelectrolytes have increased phlogistic potential that is at least in part mediated by enhanced generation of oxygen-derived metabolites and not by increased enzyme secretion or by increased fixation of complement.

• Research Organization: Univ. of Michigan, Ann Arbor
• OSTI ID: 7186451
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
ANIONS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CATIONS
CHARGED PARTICLES
CHEMILUMINESCENCE
ELECTROLYTES
ENZYMES
IMMUNE REACTIONS
IN VITRO
IN VIVO
INJURIES
IONS
LEUKOCYTES
LUMINESCENCE
MAMMALS
MATERIALS
METABOLITES
NEUTROPHILS
OXIDOREDUCTASES
RADICALS
RATS
RODENTS
SUPEROXIDE DISMUTASE
SUPEROXIDE RADICALS
TISSUES
VERTEBRATES