Differential generation of superoxide radical by rat phagocytes stimulated with IgA and IgG immune complexes

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5006974

Warren, P A; Ward, P A; Johnson, K J

Rat glycogen elicited peritoneal neutrophils and bronchoalveolar lavage (BAL) marcophages were stimulated with preformed immune complexes consisting of monoclonal IgG anti-dinitorphenol linked bovine serum albumin (DNP-BSA) or monoclonal IgA anti-DNP-BSA. Superoxide (O/sub 2//sup tau/) was detected using superoxide dismutase inhibitable reduction of ferricytochrome C. Neutrophils (2 x 10/sup 6/) generated small (<2.3 nmole/30 min.) quantities of O/sub 2//sup tau/ when stimulated with IgA-DNP-BAS in the presence or absence of cytochalasin B (1.25 ..mu..g/ml). There was a 4 to 8-fold, dose-dependent increase in O/sub 2//sup tau/ generation by neutrophils stimulated with IgG-DNP-BSA. Cytochalasin B was required to generate this quantity (up to 7.7 nmol/30 min.) of O/sub 2//sup tau/. Whether stimulated with IgG-DNP-BSA or IgA-DNP-BSA, BAL macrophages (5 x 10/sup 5/) generated more O/sub 2//sup tau/ in the absence of cytochalasin B than in its presence. Increased O/sub 2//sup tau/ generation by the immune complex stimulated BAL macrophages was dose-dependent and increased to 6-fold with IgG-DNP-BSA stimulated cells and up to 4-fold with IgA-DNP-BSA stimulated cells. These data corroborate published in vivo studies suggesting that IgA immune complex mediated tissue injury may be mediated by macrophages and not neutrophils. These data also suggest that the mechanisms for immune complex induced O/sub 2//sup tau/ generation may vary depending both on the phagocyte as well as on the stimulus.

• Research Organization: Univ. of Michigan Medical School, Ann Arbor
• OSTI ID: 5006974
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550900* -- Pathology
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
ENZYMES
GLOBULINS
IMMUNE REACTIONS
IMMUNOGLOBULINS
LAVAGE
LEUKOCYTES
MACROPHAGES
MAMMALS
MATERIALS
MONOCLONAL ANTIBODIES
NEUTROPHILS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PHAGOCYTES
PHAGOCYTOSIS
PROTEINS
RADICALS
RATS
RODENTS
SOMATIC CELLS
SUPEROXIDE DISMUTASE
SUPEROXIDE RADICALS
SYNTHESIS
VERTEBRATES