Role of macrophages and oxygen radicals in IgA induced lung injury in the rat
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:7021969
Acute lung injury in the rat has been induced by the instillation of affinity-purified mouse monoclonal IgA antibody with specific reactivity to dinitrophenol (DNP) coupled to albumin. This model of lung injury requires an intact complement system but not neutrophils, and evidence suggests that pulmonary macrophages are the critical effector cell. Macrophages retrievable from the lungs of the IgA immune complex treated rats are considerably increased in number as compared to control animals which received only the antibody. In addition these cells show evidence of activation in vivo with greater spontaneous generation of the superoxide anion (O/sub 2//sup -/) as well as significantly enhanced O/sub 2//sup -/ response in the presence of a second stimulus. Inhibition studies in vivo suggest that the lung injury is mediated by oxygen radical generation by the pulmonary macrophages. Pretreatment of rats with superoxide dismutase (SOD), catalase, the iron chelator deferoxamine or the hydroxyl radical scavenger dimethyl sulfoxide (DMSO) all markedly suppressed the development of the lung injury. In summary, these studies suggest that IgA immune complex injury in the rat lung is mediated by oxygen radical formation from pulmonary macrophages.
- Research Organization:
- Univ. of Michigan Medical School, Ann Arbor
- OSTI ID:
- 7021969
- Report Number(s):
- CONF-8604222-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
AROMATICS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MODELS
BODY
CATALASE
CONNECTIVE TISSUE CELLS
DMSO
ENZYMES
FUNCTIONS
HYDROXY COMPOUNDS
INJURIES
LUNGS
MACROPHAGES
MAMMALS
MONOCLONAL ANTIBODIES
NITRO COMPOUNDS
NITROPHENOL
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEROXIDASES
PHAGOCYTES
PHENOLS
RADICALS
RATS
RESPIRATORY SYSTEM
RESPONSE MODIFYING FACTORS
RODENTS
SOMATIC CELLS
SULFOXIDES
SUPEROXIDE DISMUTASE
SUPEROXIDE RADICALS
TOXICITY
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
AROMATICS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MODELS
BODY
CATALASE
CONNECTIVE TISSUE CELLS
DMSO
ENZYMES
FUNCTIONS
HYDROXY COMPOUNDS
INJURIES
LUNGS
MACROPHAGES
MAMMALS
MONOCLONAL ANTIBODIES
NITRO COMPOUNDS
NITROPHENOL
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEROXIDASES
PHAGOCYTES
PHENOLS
RADICALS
RATS
RESPIRATORY SYSTEM
RESPONSE MODIFYING FACTORS
RODENTS
SOMATIC CELLS
SULFOXIDES
SUPEROXIDE DISMUTASE
SUPEROXIDE RADICALS
TOXICITY
VERTEBRATES