New methods and instrumentation for the characterization of biopolymers using electrospray ionization-mass spectrometry

Conference · Tue Sep 01 04:00:00 EDT 1992

OSTI ID:7183263

Smith, R D; Udseth, H R; Rockwood, A L; Winger, B E; Hofstadler, S A; Goodlett, D R; Light-Wahl, K J

The technique of electrospray ionization (ESI) has significantly extended the ability to characterize large molecules by mass spectrometry. Proteins to at least 200,000 D can be transferred intact to the gas phase and molecular weights determined with precisions as high as 0.001% if individual charge states can be resolved. The ESI-MS can also serve as a near ideal interface and detector for capillary column separations providing a basis for highly efficient sample utilization. Using capillary electrophoresis (CE)-MS, injection quantities in the 10[sup [minus]18] mole range can be detected for smaller polypeptides using selected ion monitoring, and separation efficiencies as high as 5[center dot]10[sup 5] theoretical plates have been realized. We have recently shown that the use of small 5 [mu]m i.d. capillaries allows CE-MS with scanning detection for proteins for injection of 600 attomoles.

• Research Organization: Pacific Northwest Lab., Richland, WA (United States)
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 7183263
• Report Number(s): PNL-SA-20758; CONF-9209283--1; ON: DE93005100
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
CARBOXYLIC ACIDS
ELECTROPHORESIS
GLOBINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IONIZATION
MASS SPECTROSCOPY
MYOGLOBIN
NUCLEIC ACIDS
OLIGONUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
SPECTROSCOPY