New methods and instrumentation for the characterization of biopolymers using electrospray ionization-mass spectrometry
The technique of electrospray ionization (ESI) has significantly extended the ability to characterize large molecules by mass spectrometry. Proteins to at least 200,000 D can be transferred intact to the gas phase and molecular weights determined with precisions as high as 0.001% if individual charge states can be resolved. The ESI-MS can also serve as a near ideal interface and detector for capillary column separations providing a basis for highly efficient sample utilization. Using capillary electrophoresis (CE)-MS, injection quantities in the 10[sup [minus]18] mole range can be detected for smaller polypeptides using selected ion monitoring, and separation efficiencies as high as 5[center dot]10[sup 5] theoretical plates have been realized. We have recently shown that the use of small 5 [mu]m i.d. capillaries allows CE-MS with scanning detection for proteins for injection of 600 attomoles.
- Research Organization:
- Pacific Northwest Lab., Richland, WA (United States)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 7183263
- Report Number(s):
- PNL-SA-20758; CONF-9209283-1; ON: DE93005100
- Resource Relation:
- Conference: Mass Spectrometry Society of Japan, Japanese Society for Biomedical Mass Spectrometry conference, Osaka (Japan), 20-24 Sep 1992
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PROTEINS
MASS SPECTROSCOPY
ELECTROPHORESIS
IONIZATION
MYOGLOBIN
OLIGONUCLEOTIDES
CARBOXYLIC ACIDS
GLOBINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
SPECTROSCOPY
550200* - Biochemistry