New methods and instrumentation for the characterization of biopolymers using electrospray ionization-mass spectrometry

Conference · Tue Sep 01 04:00:00 EDT 1992

OSTI ID:10116119

Smith, R D; Udseth, H R; Rockwood, A L; Winger, B E; Hofstadler, S A; Goodlett, D R; Light-Wahl, K J

The technique of electrospray ionization (ESI) has significantly extended the ability to characterize large molecules by mass spectrometry. Proteins to at least 200,000 D can be transferred intact to the gas phase and molecular weights determined with precisions as high as 0.001% if individual charge states can be resolved. The ESI-MS can also serve as a near ideal interface and detector for capillary column separations providing a basis for highly efficient sample utilization. Using capillary electrophoresis (CE)-MS, injection quantities in the 10{sup {minus}18} mole range can be detected for smaller polypeptides using selected ion monitoring, and separation efficiencies as high as 5{center_dot}10{sup 5} theoretical plates have been realized. We have recently shown that the use of small 5 {mu}m i.d. capillaries allows CE-MS with scanning detection for proteins for injection of 600 attomoles.

• Research Organization: Pacific Northwest Lab., Richland, WA (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 10116119
• Report Number(s): PNL-SA--20758; CONF-9209283--1; ON: DE93005100
• Country of Publication: United States
• Language: English

Related Subjects

550200
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMISTRY
ELECTROPHORESIS
IONIZATION
MASS SPECTROSCOPY
MYOGLOBIN
OLIGONUCLEOTIDES
PROTEINS