New methods and instrumentation for the characterization of biopolymers using electrospray ionization-mass spectrometry
The technique of electrospray ionization (ESI) has significantly extended the ability to characterize large molecules by mass spectrometry. Proteins to at least 200,000 D can be transferred intact to the gas phase and molecular weights determined with precisions as high as 0.001% if individual charge states can be resolved. The ESI-MS can also serve as a near ideal interface and detector for capillary column separations providing a basis for highly efficient sample utilization. Using capillary electrophoresis (CE)-MS, injection quantities in the 10{sup {minus}18} mole range can be detected for smaller polypeptides using selected ion monitoring, and separation efficiencies as high as 5{center_dot}10{sup 5} theoretical plates have been realized. We have recently shown that the use of small 5 {mu}m i.d. capillaries allows CE-MS with scanning detection for proteins for injection of 600 attomoles.
- Research Organization:
- Pacific Northwest Lab., Richland, WA (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 10116119
- Report Number(s):
- PNL-SA-20758; CONF-9209283-1; ON: DE93005100
- Resource Relation:
- Conference: Mass Spectrometry Society of Japan and Japanese Society for Biomedical Mass Spectrometry conference,Osaka (Japan),20-24 Sep 1992; Other Information: PBD: Sep 1992
- Country of Publication:
- United States
- Language:
- English
Similar Records
Capillary electrophoresis-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry for direct analysis of cellular proteins
Attomole level capillary electrophoresis-mass spectrometric protein analysis using 5-[mu]m-i. d. capillaries