Attomole level capillary electrophoresis-mass spectrometric protein analysis using 5-[mu]m-i. d. capillaries

Journal Article · Tue Dec 15 04:00:00 EST 1992 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00048a019· OSTI ID:6827270

Wahl, J H; Goodlett, D R; Udseth, H R; Smith, R D ^[1]

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1. Pacific Northwest Lab., Richland, WA (United States)

In this correspondence the authors report the use of chemically modified 5-[mu]-i.d. capillaries for CE-MS of proteins. The authors have found that the use of small inner diameter capillaries results in greatly improved sensitivity in CE-ESI/MS, an approximately 25-50-fold improvement, for the detection of attomole quantities of injected protein. Previously, Moseley et al. have demonstrated femtomole level detection for peptide separation using approximately 15-[mu]m-i.d. capillaries where a continuous-flow fast atom bombardment interface was utilized, but the choice of capillary i.d. was largely dictated by their interface design, no particular sensitivity advantage related to capillary i.d. was suggested, and the ionization method used is inappropriate for proteins. To the authors knowledge this report demonstrates the first attomole range CE-MS results for proteins and, in particular, the first obtained with scanning MS detection. 19 refs., 2 figs.

• DOE Contract Number: AC06-76RL01830
• OSTI ID: 6827270
• Journal Information: Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 64:24; ISSN 0003-2700; ISSN ANCHAM
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
CARBON-OXYGEN LYASES
CARBONIC ANHYDRASE
CARBOXYLIC ACIDS
CYTOCHROMES
DATA
ELECTROPHORESIS
ENZYMES
EXPERIMENTAL DATA
GLOBINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDRO-LYASES
INFORMATION
LYASES
MASS SPECTROSCOPY
MYOGLOBIN
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
SPECTROSCOPY