Capillary electrophoresis - electrospray ionization mass spectrometry in small diameter capillaries

Conference · Mon Jun 01 04:00:00 EDT 1992

OSTI ID:7197678

Wahl, J H; Goodlett, D R; Udseth, H R; Smith, R D

Methods (such as small inner diameter capillaries) are being explored to increase analyte sensitivity in capillary electrophoresis- electrospray ionization/mass spectroscopy(CE-ESI/MS). Results are reported for melittin in a protein mixture, with 10 to 100 {mu}m ID capillaries; and for a mixture of aprotinin, cytochrome c, myoglobin, and carbonic anhydrase, with 5 to 50 {mu}m ID capillaries. It is shown that an increase in solute sensitivity occurs when small ID capillaries ({lt} 20 {mu}m) are used in CE-ESI/MS for both a peptide and a protein mixture. 3 figs. (DLC)

• Research Organization: Pacific Northwest Lab., Richland, WA (United States)
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 7197678
• Report Number(s): PNL-SA-20440; CONF-9205196--6; ON: DE92019123
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105 -- Separation Procedures
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BLOOD VESSELS
BODY
CAPILLARIES
CARBON-OXYGEN LYASES
CARBONIC ANHYDRASE
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CYTOCHROMES
DISPERSIONS
ELECTROPHORESIS
ENZYMES
GLOBINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDRO-LYASES
LYASES
MASS SPECTROSCOPY
MIXTURES
MYOGLOBIN
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDES
PIGMENTS
PORPHYRINS
PROTEINS
SENSITIVITY
SIZE
SPECTROSCOPY