Combined capillary electrophoresis and electrospray ionization mass spectrometry

Conference · Fri Sep 01 00:00:00 EDT 1989

OSTI ID:5415500

Smith, R. D.; Loo, J. A.; Barinaga, C. J.; Edmonds, C. G.; Udseth, H. R.

The special capabilities of the capillary electrophoresis electrospray ionization-mass spectrometer interface for the analysis of peptides and proteins with molecular weights extending to in excess of 100 kilodalton are demonstrated. The dynamic combinations of both capillary zone electrophoresis and capillary isotachophoresis with electrospray ionization are illustrated for mixtures of peptides and proteins. The potential extension of these methods for determination of the primary structure (sequence) of proteins using tandem mass spectrometry is shown to be facilitated by the high charge state of ions produced by the electrospray interface. The relevance of these results for advances in analytical biochemistry is discussed. 24 refs., 4 figs.

• Research Organization: Pacific Northwest Lab., Richland, WA (USA)
• Sponsoring Organization: DOE/NE
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 5415500
• Report Number(s): PNL-SA-17257; CONF-8909252--1; ON: DE90001903
• Country of Publication: United States
• Language: English

Related Subjects

440300* -- Miscellaneous Instruments-- (-1989)
47 OTHER INSTRUMENTATION
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMISTRY
CAPILLARY FLOW
CHEMISTRY
CHROMATOGRAPHY
CONTROL SYSTEMS
DATA
DEMONSTRATION PROGRAMS
DIGESTION
DYNAMIC MASS SPECTROMETERS
ELECTROPHORESIS
EXPERIMENTAL DATA
FLUID FLOW
IMMOBILIZED ENZYMES
INFORMATION
INTERACTIONS
IONIZATION
ISOTACHOPHORESIS
MASS SPECTROMETERS
MASS SPECTROSCOPY
MEASURING INSTRUMENTS
MEASURING METHODS
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
PEPTIDES
PROTEINS
SEPARATION PROCESSES
SPECTROMETERS
SPECTROSCOPY