Photoeffects of near ultraviolet light upon a polycyclic aromatic hydrocarbon exposed to mouse skin microsomes
Near ultraviolet (UV) light has been reported to both enhance and inhibit the tumor incidence in mice dermally exposed to benzo(a)pyrene (BaP) or polycyclic aromatic hydrocarbon (PAH) mixtures. Near UV light interacts with PAHs producing a variety of oxygenated products such as phenols, endoperoxides and quinones. However, little is known about BaP products formed from near UV irradiation of BaP-exposed mouse skin. Therefore, [sup 14]C-BaP was incubated with 3-methylcholanthrene (3-MC) induced C[sub 3]H/HeJ and DBA/2J mouse skin microsomes with or without a 365 nm light source. The results indicated that the concurrent 365 nm light irradiation of induced mouse skin microsomes and BaP greatly enhanced the total conversion of BaP to its products, approximately 3-fold for the C[sub 3]H/HeJ and approximately 7-fold for the DBA/2J mouse microsomes, compared to the induced mouse skin microsomes and BaP alone. HPLC analyses of organic extracts indicated a more than additive enhancement of the formation of most of the individual cochromatographed BaP metabolites due to the combined interaction of 365 nm light with BaP and skin microsomes. Similar interactions were observed using benz(a)anthracene (BaA) in this system. These data show that near UV light alters the metabolic profile of PAHs produced by mouse skin microsomes.
- Research Organization:
- Cincinnati Univ., OH (United States)
- OSTI ID:
- 7174996
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARCINOGENESIS
CELL CONSTITUENTS
CONDENSED AROMATICS
DISPERSIONS
ELECTROMAGNETIC RADIATION
HYDROCARBONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MICE
MICROSOMES
MIXTURES
NEAR ULTRAVIOLET RADIATION
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
POLYCYCLIC AROMATIC HYDROCARBONS
RADIATION EFFECTS
RADIATIONS
RIBOSOMES
RODENTS
SKIN
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
VERTEBRATES