Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Localization of radiolabeled mouse alloantibody in a sarcoma induced by 3-methylcholanthrene

Journal Article · · J. Nucl. Med.; (United States)
OSTI ID:5287516
The distribution of twice-purified I-125-labeled alloantibody, prepared from the serum of strain DBA/2J mice obtained after immunization with strain C3H/HeJ spleen cells, was studied in immunosuppressed DBA/2J mice bearing either an allogeneic C3H/HeJ MCA sarcoma (i.e., one induced by 3-methylcholanthrene) or a syngeneic MCA sarcoma. Radiolabeled antibody was isolated from labeled immune gamma globulin by adsorption onto C3H/HeJ red blood cells and elution from stroma prepared from these cells, followed by Sephadex G-200 gel filtration chromatography. This purified antibody bound specifically in vitro to C3H/HeJ red blood cells. In vivo this antibody localized preferentially in C3H/HeJ MCA sarcomas. An enrichment procedure was developed to increase the yield of radiolabeled alloantibody. The localization of I-131-labeled alloantibody in allogeneic tumor was visualized by external scintigraphy. Control I-131-labeled normal DBA/2J gamma globulin did not show such tumor localization.
Research Organization:
Univ. of Minnesota, Minneapolis
OSTI ID:
5287516
Journal Information:
J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 21:1; ISSN JNMEA
Country of Publication:
United States
Language:
English