Localization of radiolabeled antibody in SVT2 tumor increases with immunosuppression of the host. [Mice]

Journal Article · Fri Oct 01 04:00:00 EDT 1982 · Proc. Soc. Exp. Biol. Med.; (United States)

DOI:https://doi.org/10.3181/00379727-171-41477· OSTI ID:5596917

Buchsbaum, D J; Anderson, J M; Bray, B E

The localization of radiolabeled tumor-specific antibodies to an SV40-transformed mouse tumor was analyzed in immunosuppressed (X-ray and cortisone) and nonimmunosuppressed mice. (C57B1/6 x Balb/c)F/sub 1/ mice were immunized with the SVT2 tumor of Balb/c origin. Radiolabeled antibody was isolated from /sup 125/I-labeled immune gamma globulin by adsorption onto SVT2 cells, and elution from these cells using citrate buffer. The radiolabeled antibodies were injected into normal (C57B1/6 x Balb/c)F/sub 1/ mice. The purified antibodies present in this serum bound specifically in vitro to SV40-transformed cell lines. In vivo, the /sup 125/I-labeled antibodies localized preferentially in the SVT2 tumor in immunosuppressed mice. Significantly less /sup 125/I-labelled antibody localized in the SVT2 tumor in nonimmunosuppressed mice.The localization of /sup 125/I-labeled antibody in SVT2 tumor in immunosuppressed mice was reduced significantly by passive administration of anti-SVT2 serum.

• Research Organization: Univ. of Minnesota, Minneapolis
• OSTI ID: 5596917
• Journal Information: Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 171:1; ISSN PSEBA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL LOCALIZATION
BIOLOGICAL RADIATION EFFECTS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
HALOGEN COMPOUNDS
IMMUNE REACTIONS
IMMUNOSUPPRESSION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
MAMMALS
MICE
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RODENTS
TRACER TECHNIQUES
VERTEBRATES
X RADIATION