Leukotriene B/sub 4/ modulates human polymorphonuclear leukocyte (PMN) phospholipid (PL) methylation
Formation of phosphatidylcholine (PC) by methylation of phosphatidylethanolamine (PE) is required for transduction of chemotactic factor messages in phagocytic cells, including the development of a motile configuration and release of arachidonic acid (AA) from PL. The authors examined PL methylation in human peripheral blood PMN following stimulation by leukotriene B/sub 4/ (LTB/sub 4/), a potent chemotactic lipid metabolite of AA that is produced by human PMN. (/sup 3/H)-methionine, in the presence or absence of LTB/sub 4/, was added directly to the cells without a preloading period. (/sup 3/H)-methyl mono- and di-methylated PE, PC and lyso PC were separated by thin layer chromatography. PL methylation in human PMN is dependent on time of incubation, LTB/sub 4/ concentration and methionine concentration. The optimal LTB/sub 4/ concentration is 10/sup -7/ M, the same concentration that induces a maximal chemotactic response in PMN. At early time points (2-10 min), formation of methylated PL is enhanced following LTB/sub 4/ stimulation. In contrast, at later time points (20-60 min), methylated PL synthesis in LTB/sub 4/ treated cells is depressed or the same as control cells. Synthesis of PC by the choline transferase pathway is not affected by LTB/sub 4/ stimulation. This data shows that, at early time points following LTB/sub 4/ stimulation, PL methylation is enhanced. This correlates with other reports of calcium mobilization and chemotaxis in PMN at early time points following LTB/sub 4/ stimulation.
- Research Organization:
- Univ. of Pennsylvania, Philadelphia
- OSTI ID:
- 7171389
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
ARACHIDONIC ACID
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ESTERS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
LIPIDS
LIPOTROPIC FACTORS
MAMMALS
MAN
MATERIALS
METABOLITES
METHIONINE
METHYLATION
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHOLIPIDS
PRIMATES
REACTION KINETICS
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY
TIME DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES