Leukotriene B/sub 4/ (LTB/sub 4/) induces formation of inositol-phosphates (IP's) in rat peritoneal polymorphonuclear leukocytes (PMN's)

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5268455

Chi-Rosso, G; Crooke, S T; Mong, S

LTB/sub 4/ induced rapid breakdown of prelabeled inositol-phospholipids (PI) in rat PMN. Formation of (/sup 3/H)-inositol-trisphosphate ((/sup 3/H)-IP/sub 3/) was rapid, with a peak of 250-300% of the control level, after 5-15 sec of stimulation with LTB/sub 4/. Accumulation of (/sup 3/H)-inositol-bisphosphate ((/sup 3/H)-IP/sub 2/) was rapid, peaking after 30 sec of stimulation. (/sup 3/H)-inositol-monophosphate ((/sup 3/H)-IP/sub 1/) accumulated gradually in the presence of LiCl. The kinetics of (/sup 3/H)-IP/sub 3/, (/sup 3/H)-IP/sub 2/ and (/sup 3/H)-IP/sub 1/ accumulation suggested that LTB/sub 4/ may interact with receptors in PMNs, activate phospholipase C which, in turn, induces hydrolysis of PI. The agonist activities of several LTB/sub 4/ analogs were employed to investigate the structure activity relationship of LTB/sub 4/ receptor mediated activation of PI hydrolysis. Increases in (/sup 3/H)-IP/sub 3/ formation were dependent upon the concentration of LTB/sub 4/ and the agonist analogs. The rank order potency of these analogs were equivalent to that of the pharmacological activity of LTB/sub 4/ agonists in the chemotaxis assay. Furthermore, the Islet activation protein (IAP) inhibited LTB/sub 4/ induced (/sup 3/H)-IP/sub 3/ formation. The tumor promoting phorbomyristate ester also inhibited LTB/sub 4/ induced (/sup 3/H)-IP/sub 3/ formation. These results suggest LTB/sub 4/ may interact with receptors in rat PMNs, activate G/sub i/ protein regulated phospholipase C and induce (/sup 3/H)-IP/sub 3/ formation.

• Research Organization: SKF Labs., Philadelphia, PA
• OSTI ID: 5268455
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ARACHIDONIC ACID
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBOXYLIC ACIDS
CARCINOGENS
ESTERS
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHORBOL ESTERS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SACCHARIDES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES