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Activation of the lipoxygenase pathway in the methionine enkephalin induced respiratory burst in human polymorphonuclear leukocytes

Journal Article · · Life Sci.; (United States)
; ; ; ;

In comparative studies of f-met-Leu-Phe (FMLP) and methionine enkephalin (ME) induced polymorphonuclear leukocyte (PMNL) stimulation the following results were obtained: (i) both FMLP and ME increased the intracellular killing (IK) capability of human PMNLs probably through NADPH oxidase activation, (ii) the ME-induced respiratory burst (RB) differed from the chemotactic peptide FMLP-triggered superoxide generation because the former was not accompanied by the activation of the glutathione system and the duration of the superoxide production was prolonged. The reaction was dependent on lipoxygenation, was potentiated by indomethacin (IM) and was inhibited by nordihidro-guairetic acid (NDGA), (iii) both /sup 14/C-arachidonic acid release and leukotriene B/sub 4/ (LTB/sub 4/) synthesis of ME-treated PMNLs were elevated as compared to those of FMLP triggered cells. Their results suggest that lipoxygenation and even an increased LTB/sub 4/ synthesis are involved in the ME-induced RB of leukocytes.

Research Organization:
Univ. Medical School, Debrecen (Hungary)
OSTI ID:
7143566
Journal Information:
Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 42:22; ISSN LIFSA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARACHIDONIC ACID
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
DRUGS
ENDORPHINS
ENKEPHALINS
ENZYME ACTIVITY
ENZYMES
FUNCTIONS
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MAN
MATERIALS
MONOCARBOXYLIC ACIDS
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PEPTIDES
POLYPEPTIDES
PRIMATES
PROTEINS
SYNTHESIS
VERTEBRATES