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Chemotactic peptide-induced arachidonic acid mobilization in human polymorphonuclear leukocytes

Journal Article · · Am. J. Pathol.; (United States)
OSTI ID:6600567

Human polymorphonuclear leukocytes prelabeled with tritiated arachidonic acid liberated radiolabeled products when exposed to the chemotactic peptide fMet-Leu-Phe. The effect was enhanced in the presence of phorbol-12-myristate 13-acetate or 1-oleoyl-2-acetyl glycerol; these agents activate phospholipid- and Ca2+-dependent protein kinase C. In contrast, arachidonic acid mobilization was suppressed by two compounds known to inhibit protein kinase C activity: polymyxin B and 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine. These results suggest that protein kinase C was involved in arachidonic acid mobilization in leukocytes stimulated with chemotactic peptide.

Research Organization:
Medical Univ. of South Carolina, Charleston (USA)
OSTI ID:
6600567
Journal Information:
Am. J. Pathol.; (United States), Journal Name: Am. J. Pathol.; (United States) Vol. 133:2; ISSN AJPAA
Country of Publication:
United States
Language:
English

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