Identification and characterization of monoclonal antibodies specific for macrophages at intermediate stages in the tumoricidal activation pathway

Journal Article · Mon Jan 15 04:00:00 EST 1990 · Journal of Immunology; (USA)

OSTI ID:7167611

Paulnock, D M; Lambert, L E ^[1]

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1. Univ. of Wisconsin Medical School, Madison (USA)

Macrophage activation for tumor cell killing is a multistep pathway in which responsive macrophages interact sequentially with priming and triggering stimuli in the acquisition of full tumoricidal activity. A number of mediators have been identified which have activating capability, including in particular IFN-gamma and bacterial LPS. Although the synergistic functional response of normal macrophages to sequential incubation with these activation signals has been well-established, characterization of the intermediate stages in the activation pathway has been difficult. We have developed a model system for examination of various aspects of macrophage activation, through the use of the murine macrophage tumor cell line, RAW 264.7. These cells, like normal macrophages, exhibit a strict requirement for interaction with both IFN-gamma and LPS in the development of tumor cytolytic activity. In addition, these cells can be stably primed by the administration of gamma-radiation. In the studies reported here, we have used RAW 264.7 cells treated with IFN-gamma alone or with IFN-gamma plus LPS to stimulate the production of rat mAb probes recognizing cell surface changes occurring during the activation process. In this way we have identified three Ag associated with intermediate stages of the activation process. One Ag, TM-1, is expressed on RAW 264.7 cells primed by IFN-gamma or gamma-radiation. This surface Ag thus identifies cells at the primed cell intermediate stage of the tumoricidal activation pathway regardless of the mechanism of activation. A second Ag, TM-2, is expressed on IFN-treated RAW 264.7 cells but not on RAW 264.7 cells primed with gamma-radiation alone. Expression of this Ag can be induced by treatment of irradiated cells with IFN-gamma, but is not induced by IFN-gamma treatment of a noncytolytic cell line, WEHI-3.

• OSTI ID: 7167611
• Journal Information: Journal of Immunology; (USA), Journal Name: Journal of Immunology; (USA) Vol. 144:2; ISSN JOIMA; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOLOGICAL PATHWAYS
BIOLOGICAL RADIATION EFFECTS
CARBOHYDRATES
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ELECTROMAGNETIC RADIATION
FUNCTIONS
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INTERFERON
IONIZING RADIATIONS
LIPIDS
LIPOPOLYSACCHARIDES
LYMPHOKINES
MACROPHAGES
MAMMALS
MEMBRANES
MICE
MITOGENS
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
PERITONEUM
PHAGOCYTES
POLYSACCHARIDES
PROTEINS
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RADIATIONS
RODENTS
SACCHARIDES
SEROUS MEMBRANES
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