Modulation of macrophage function by gamma-irradiation. Acquisition of the primed cell intermediate stage of the macrophage tumoricidal activation pathway

Journal Article · Thu Oct 15 04:00:00 EDT 1987 · J. Immunol.; (United States)

OSTI ID:5849035

Lambert, L E; Paulnock, D M

Macrophage activation for tumor cell killing is a multistep pathway in which responsive macrophages interact sequentially with priming and triggering stimuli in the acquisition of full tumoricidal activity. Although this synergistic response of normal macrophages to sequential incubation with activation signals has been well established, characterization of the intermediate stages in this pathway has been difficult, due in large measure to the instability of the intermediate cell phenotypes. We have developed a model system for examination of macrophage-mediated tumor cell lysis, with the use of the murine macrophage tumor cell line RAW 264.7. These cells, like normal macrophages, exhibit a strict requirement for interaction with both interferon-gamma (IFN-gamma, the priming signal) and bacterial lipopolysaccharide (LPS, the triggering signal) in the development of tumor cytolytic activity. In this system, the priming effects of IFN-gamma decay rapidly after withdrawal of this mediator and the cells become unresponsive to LPS triggering. We have recently observed that gamma-irradiation of the RAW 264.7 cells also results in development of a primed activation state for tumor cell killing. The effects of gamma-radiation on the RAW 264.7 cell line are strikingly similar to those resulting from incubation with IFN-gamma, with the exception that the irradiation-induced primed cell intermediate is stable and responsive to LPS triggering for at least 24 hr. Treatment with gamma-radiation also results in increased cell surface expression of major histocompatibility complex-encoded class I antigens; however, class II antigen expression is not induced.

• Research Organization: Univ. of Wisconsin Medical School, Madison
• OSTI ID: 5849035
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 139:8; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MODELS
BIOLOGICAL PATHWAYS
BIOLOGICAL RADIATION EFFECTS
CARBOHYDRATES
CELL DIVISION
CELL KILLING
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
FUNCTIONS
GAMMA RADIATION
GROWTH FACTORS
INTERFERON
IONIZING RADIATIONS
LIPIDS
LIPOPOLYSACCHARIDES
LYMPHOKINES
LYSIS
MACROPHAGES
MAMMALS
MICE
MITOGENS
ORGANIC COMPOUNDS
PHAGOCYTES
POLYSACCHARIDES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RODENTS
SACCHARIDES
SOMATIC CELLS
TUMOR CELLS
VERTEBRATES