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Induction of macrophage antitumor activity by gamma radiation

Journal Article · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6963437
;
The authors have developed a model system for examination of macrophage-mediated tumor cells lysis, using the murine macrophage tumor cell line RAW 264.7. These cells, like normal macrophages, exhibit a strict requirement for interaction with both interferon-..gamma.. (IFN-..gamma.., the priming signal) and bacterial lipopolysaccharide (LPS, the triggering signal) in the development of tumor cytolytic activity. In this system, the priming effects of IFN-..gamma.. decay rapidly following withdrawal of this mediator and the cells become unresponsive to LPS. They have recently observed that gamma radiation of the RAW 264.7 cells results in development of a primed state which is stable and responsive to LPS triggering for a least 48 hours. Irradiation-induced development of the primed phenotype is not solely the result of cytostatic effects as LPS treatment alone results in marked decreases in /sup 3/H-TdR incorporation in the absence of cytolytic potential. In addition to delivering the priming signal for tumor cytotoxicity, irradiation of this cell line results in changes in cell morphology that are typical of activation. Finally, treatment with irradiation results in increased cell surface expression of MHC-encoded Class I antigens; however, Class II antigen expression is not induced. Thus, the effects of gamma radiation on this cell line are strikingly similar to those resulting from incubation with IFN-..gamma...
Research Organization:
Univ. of Wisconsin School of Medicine, Madison
OSTI ID:
6963437
Report Number(s):
CONF-8604222-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MODELS
BIOLOGICAL RADIATION EFFECTS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
GROWTH FACTORS
INTERFERON
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LIPOPOLYSACCHARIDES
LYMPHOKINES
LYSIS
MACROPHAGES
MAMMALS
MICE
MITOGENS
ORGANIC COMPOUNDS
PHAGOCYTES
PHENOTYPE
POLYSACCHARIDES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RODENTS
SACCHARIDES
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES