Analysis of blood biomarkers from bluegill sunfish
- Western North Carolina Univ., Cullowhee (USA)
- Oak Ridge National Lab., TN (USA)
The test animal for this project was a hybrid of the bluegill sunfish, Lepomis machrochirus. These fish were lab-reared and maintained in large, flow-through tanks at 10 C. The chemical benzo(a)pyrene (BaP) was used in the experiments. It is a carcinogen found in the environment most often as a by product of combustion of organic material. BaP is also an inducer of the mixed function oxidase system, which is the organisms detoxification system and which produces metabolites of the original chemicals. It is these metabolites which have the potential to interact with the DNA. The toxicant was present in the water at approximately 1 ppb. This was obtained by passing water through a generator column of glass beads, to which BaP had been adsorbed. This was a long-term, low level chronic exposure. Two procedures were used to evaluate the effects of BaP on the fish. The DNA of the livers was examined for strand breaks by the alkaline unwinding assay and the nuclei of the whole blood cells were examined for abnormalities by flow cytometry.
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 7165501
- Report Number(s):
- CONF-8909236--
- Journal Information:
- Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA), Journal Name: Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA) Vol. 28:2; ISSN 0099-7293; ISSN ACWCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
540320* -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BENZOPYRENE
BIOLOGICAL INDICATORS
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
CELL FLOW SYSTEMS
CHRONIC EXPOSURE
CONDENSED AROMATICS
DIGESTIVE SYSTEM
DNA
ECOLOGICAL CONCENTRATION
ENZYME INDUCTION
ENZYMES
FISHES
GENE REGULATION
GLANDS
HYDROCARBONS
LIVER
MATERIALS
METABOLITES
MIXED-FUNCTION OXIDASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
POLLUTION
SENSITIVITY
STRAND BREAKS
TOXICITY
VERTEBRATES
WATER POLLUTION