Laboratory and field studies on biotransformation enzymes in sunfish (Lepomis sp. )

Conference · Thu Sep 01 04:00:00 EDT 1988 · Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA)

OSTI ID:7165522

Jimenez, B D; Oikari, A; Adams, S M; Esau, B D; Morrissey, C M; Petanen, T I ^[1]

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1. Oak Ridge National Lab., TN (USA)

To cope with pollutants, organisms synthesize enzymes that detoxify organic compounds (mixed function oxidases or MFOs). A series of these enzymes are presently being employed as indicators of pollution at Oak Ridge National laboratory in the Y-12 Biological Monitoring and Abatement Program. The quantities and activities of several hepatic enzymes, as well as the liver somatic indices, were measured in redbreast sunfish, Leopomis auritus, sampled from several stations along a stream receiving large inputs of industrial and municipal effluents and a stream in a non-industrialized, rural drainage area. Fish collected close to the industrial and municipal outfalls exhibited high quantities and activities of these enzymes, as well as elevated somatic indices, compared to those from the rural stream and from other locations along the polluted stream. Laboratory experiments were conducted using Bluegill-green sunfish hybrid which were exposed to approximately 0.5 g Benzo(a)pyrene (BaP) per liter under 24 h static renewal conditions. The time course of induction for MFO activity was obtained. Induction of MFO activity occurred a week after BaP exposure.

• OSTI ID: 7165522
• Report Number(s): CONF-8909236--
• Conference Information: Journal Name: Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA) Journal Volume: 28:2
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540320* -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BENZOPYRENE
BIOLOGICAL INDICATORS
BODY
CONDENSED AROMATICS
DIGESTIVE SYSTEM
ENZYME ACTIVITY
ENZYME INDUCTION
ENZYMES
FISHES
GENE REGULATION
GLANDS
HYDROCARBONS
INDUSTRIAL WASTES
LIVER
MIXED-FUNCTION OXIDASES
MUNICIPAL WASTES
NATIONAL ORGANIZATIONS
ORGANIC COMPOUNDS
ORGANS
ORNL
OXIDOREDUCTASES
OXYGENASES
POLLUTION
SENSITIVITY
TOXICITY
US AEC
US DOE
US ERDA
US ORGANIZATIONS
VERTEBRATES
WASTES
WATER POLLUTION