Toluene transposons Tn4651 and Tn4653 are class II transposons

Journal Article · Wed Mar 01 04:00:00 EST 1989 · Journal of Bacteriology; (USA)

OSTI ID:7164636

Tsuda, Masataka; Minegishi, Koh-ichi; Iino, Tetsuo ^[1]

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1. Univ. of Tokyo (Japan)

The toluene degradative transposon Tn4651 is included within another transposon, Tn4653, and both of these elements are members of the Tn3 family. The tnpA gene product of each element mediates formation of cointegrates as intermediate products of transposition, and the tnpS and tnpT gene products encoded by Tn4651 take part in resolution of both Tn4651- and Tn4653-mediated cointegrates. Sequence analysis demonstrated that Tn4651 and Tn4653 have 46- and 38-base-pair terminal inverted repeats, respectively, and that both elements generate 5-base-pair duplication of the target sequence upon transposition. Complementation test of the Tn4651 - and Tn4653-encoded transposition functions with those of Tn3, Tn21, and Tn1721 showed that (i) the trans-acting transposition functions encoded by Tn4651 were not interchangeable with those encoded by the four other transposons, (ii) the Tn4653 tnpA function was interchangeable with the Tn1721 function, and (iii) Tn4653 coded for a resolvase (tnpR gene product) that complemented the tnpr mutations of Tn21 and Tn1721. The Tn4653 tnpR gene was located just 5{prime} upstream of the tnpA gene sheared extensive sequence homology with the Tn1721 tnpR gene. The res region was located adjacent to the tnpR gene, and sequence analysis indicated that failure of the Tn4653 tnpR product to resolve the Tn4653-mediated cointegrates is ascribed to an incomplete structure of the res region.

• OSTI ID: 7164636
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 171:3; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATED AROMATICS
AMINO ACID SEQUENCE
AROMATICS
DNA SEQUENCING
DNA-ASE
ENDONUCLEASES
ENZYMES
ESTERASES
GENES
HYDROCARBONS
HYDROLASES
METABOLISM
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
STRUCTURAL CHEMICAL ANALYSIS
TOLUENE
TRANSPOSONS