Intramolecular transposition by a synthetic IS50 (Tn5) derivative

Journal Article · Thu Nov 01 04:00:00 EST 1990 · Journal of Bacteriology; (USA)

OSTI ID:5948514

Tomcsanyi, T; Phadnis, S H; Berg, D E ^[1]; Berg, C M ^[2]

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1. Washington Univ. Medical School, St. Louis, MO (USA)
2. Univ. of Connecticut, Storrs (USA)

We report the formation of deletions and inversions by intramolecular transposition of Tn5-derived mobile elements. The synthetic transposons used contained the IS50 O and I end segments and the transposase gene, a contraselectable gene encoding sucrose sensitivity (sacB), antibiotic resistance genes, and a plasmid replication origin. Both deletions and inversions were associated with loss of a 300-bp segment that is designated the vector because it is outside of the transposon. Deletions were severalfold more frequent than inversions, perhaps reflecting constraints on DNA twisting or abortive transposition. Restriction and DNA sequence analyses showed that both types of rearrangements extended from one transposon end to many different sites in target DNA. In the case of inversions, transposition generated 9-bp direct repeats of target sequences.

• OSTI ID: 5948514
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 172:11; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BACTERIA
DNA
DNA SEQUENCING
DRUGS
ENZYMES
GENES
MICROORGANISMS
MOLECULAR BIOLOGY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
STRUCTURAL CHEMICAL ANALYSIS
TRANSPOSONS