Mutagenesis of dimeric plasmids by the transposon. gamma. delta. (Tn1000)
Journal Article
·
· Journal of Bacteriology; (USA)
OSTI ID:6534210
- Univ. of Connecticut, Storrs (USA)
The Escherichia coli F factor mediates conjugal transfer of a plasmid such as pBR322 primarily by replicative transposition of transposon {gamma}{delta} (Tn1000) from F to that plasmid to form a cointegrate intermediate. Although resolution of this cointegrate always yields a plasmid containing a single {gamma}{delta} insertion, the occasional recovery of transposon-free plasmids after connuugal transfer has led to alternative hypotheses for F mobilization. The authors show here that {gamma}{delta}-free plasmids are found after F-mediated conjugal transfer only when the donor plasmid is a dimer and the recipient is Rec{sup +}.
- DOE Contract Number:
- FG02-89ER60862
- OSTI ID:
- 6534210
- Journal Information:
- Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 172:5; ISSN JOBAA; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
Similar Records
Transposition of plasmid DNA segments specifying hydrocarbon degradation and their expression in various microorganisms
A plasmid carrying mucA and mucB genes from pKM101 in Haemophilus influenzae and Escherichia coli
Toluene transposons Tn4651 and Tn4653 are class II transposons
Journal Article
·
Sat Jul 01 00:00:00 EDT 1978
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:6355443
A plasmid carrying mucA and mucB genes from pKM101 in Haemophilus influenzae and Escherichia coli
Journal Article
·
Sun Oct 01 00:00:00 EDT 1989
· Journal of Bacteriology; (USA)
·
OSTI ID:5258815
Toluene transposons Tn4651 and Tn4653 are class II transposons
Journal Article
·
Tue Feb 28 23:00:00 EST 1989
· Journal of Bacteriology; (USA)
·
OSTI ID:7164636