Interactions between cell growth-regulating domains in the products of the adenovirus E1A oncogene
Among the various biological activities expressed by the products of the adenovirus E1A gene are the abilities to induce cellular DNA synthesis and proliferation in quiescent primary baby rat kidney cells. The functional sites for these activities lie principally within two regions of the E1A proteins: an N-terminal region and a small second region of approximately 20 amino acids further downstream. To study the biological functions of the first domain, the authors constructed an in-frame deletion of amino acid positions 23 through 107 of the E1A products. This deletion did not impede the ability of the E1A products to transactivate the adenovirus early region 3 promoter in a transient-expression assay in HeLa cells. The ability to induce DNA synthesis in quiescent baby rat kidney cells was, however, lost in the absence of these sequences. Deletion of the small second region induced a form of S phase in which DNA synthesis occurred in the apparent absence of controls required for the cessation of DNA synthesis and progression through the remainder of the cell cycle. These cells did not appear to accumulate in or before G2, and many appeared to have a DNA content greater than that in G2. The functions of both domains are required for production of transformed foci in a ras cooperation assay. Focus formation occurred, however, even when the two domains were introduced on two separate plasmids. This complementation effect appeared to require expression of both of the mutant proteins and did not appear to result merely from recombination at the DNA level.
- Research Organization:
- Cold Spring Harbor Lab., Cold Spring Harbor, NY (US)
- OSTI ID:
- 7164172
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 8:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ADENOVIRUS
ONCOGENES
CELL PROLIFERATION
GENETIC CONTROL
GENE RECOMBINATION PROTEINS
DNA SEQUENCING
MUTANTS
MOLECULAR BIOLOGY
AMINO ACID SEQUENCE
DNA REPLICATION
EXPERIMENTAL DATA
GENE REGULATION
HELA CELLS
KIDNEYS
ONCOGENIC TRANSFORMATIONS
PHENOTYPE
RATS
ANIMALS
BODY
CELL TRANSFORMATIONS
CONTROL
DATA
GENES
INFORMATION
MAMMALS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEIC ACID REPLICATION
NUCLEOPROTEINS
NUMERICAL DATA
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANS
PARASITES
PEST CONTROL
PROTEINS
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
VIRUSES
550400* - Genetics
550200 - Biochemistry