Mutagenicity of soluble and insoluble nickel compounds at the gpt locus in G12 Chinese hamster cells

Journal Article · Fri Jan 01 04:00:00 EST 1993 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850210408· OSTI ID:7159078

Lee, Y W; Pons, C; Tummolo, D M; Klein, C B; Rossman, T G; Christie, N T ^[1]

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1. New York Univ. Medical Center, New York City, NY (United States)

Nickel is an established human and animal carcinogen, but efforts to demonstrate its mutagenicity in a number of cell types have not been successful. In this report the authors describe the mutational response to nickel compounds in the G12 cell line, an hprt deficient V79 cell line containing a single copy of the E. coli gpt gene. This cell line has a low spontaneous background, making it suitable for assessment of mutagenic responses to environmental contaminants. When G12 cells were treated with insoluble particles of crystalline nickel sulfide <5 [mu]m in diameter, a strong, dose-dependent mutagenic response was observed up to 80 times the spontaneous background. Of 48 mutant gpt([minus]) clones isolated that were induced by insoluble nickel, all were capable of DNA amplification of the gpt sequences by polymerase chain reaction (PCR). The ability to produce full-length PCR products is an indication that large deletions of gene sequences have not occurred. When G12 cells were treated with soluble nickel sulfate, the mutational response was not significantly increased over the spontaneous background. This difference in mutagenic response reflects a large difference in the mutagenic potential of soluble and insoluble nickel compounds, which reflects the carcinogenic potential of these forms of nickel. 45 refs., 5 figs.

• OSTI ID: 7159078
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 21:4; ISSN EMMUEG; ISSN 0893-6692
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560300* -- Chemicals Metabolism &amp; Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOASSAY
CHALCOGENIDES
DOSE-RESPONSE RELATIONSHIPS
MUTAGENESIS
NICKEL COMPOUNDS
NICKEL SULFATES
NICKEL SULFIDES
OXYGEN COMPOUNDS
SOLUBILITY
SULFATES
SULFIDES
SULFUR COMPOUNDS
TRANSITION ELEMENT COMPOUNDS