Detection of deletion mutations at the gpt locus in pSV2gpt transformed CHO cells
Technical Report
·
OSTI ID:5697997
Deletion mutations were detected at the gpt locus in HGPRT-deficient CHO cells transformed by the pSV2gpt plasmid vector. The purine analog, 6-thioguanine (TG), is metabolized by XGPRT and was shown to be applicable for the isolation of mutants at the gpt locus in one pSV2gpt transformed CHO cell line carrying a single copy of the gpt gene. Induction of TG/sup r/ mutants in this cell line by x-rays was quantifiable. Specific deletions of pSV2gpt sequences in two TG/sup r/ mutants were detected by blot hybridization. The sites of these depletions provide a molecular basis for the observed TG/sup r/ phenotype. 17 references.
- Research Organization:
- Tennessee Univ., Oak Ridge (USA). Graduate School of Biomedical Sciences; Oak Ridge National Lab., TN (USA)
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5697997
- Report Number(s):
- DOE/NBM-3015766; ON: DE83015766
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550400 -- Genetics
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
CELL CONSTITUENTS
CHO CELLS
DATA
ELECTROMAGNETIC RADIATION
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IONIZING RADIATIONS
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RADIOINDUCTION
X RADIATION
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
CELL CONSTITUENTS
CHO CELLS
DATA
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
GENES
INFORMATION
IONIZING RADIATIONS
MUTATIONS
NUMERICAL DATA
PLASMIDS
RADIATIONS
RADIOINDUCTION
X RADIATION