Transgenic Chinese hamster V79 cell lines which exhibit variable levels of gpt mutagenesis

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Environmental and Molecular Mutagenesis; (USA)

DOI:https://doi.org/10.1002/em.2850160102· OSTI ID:5734023

Klein, C B; Rossman, T G ^[1]

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1. New York Univ. Medical Center, New York (USA)

The Escherichia coli gpt gene coding for xanthine-guanine phosphoribosyl transferase has been stably transfected into HPRT{sup {minus}} Chinese hamster V79 cells. Several gpt{sup {minus}} cell lines have been established, which retain the sequence(s) even after long-term culture without selection for gpt. While spontaneous mutagenesis to gpt{sup {minus}} occurs rather frequently for most cell lines, it cannot be correlated with either the number of plasmid integration sites or deletion of the plasmid sequence(s). One transgenic cell line (g12), which continuously maintains a low spontaneous mutation frequency was used in comparative mutagenesis studies with wild-type V79 cells (gpt vs. hprt). Alkylating agents such as N-methyl-N{prime}-nitro-N-nitrosoguanidine (MNNG) and {beta}-propiolactone (BPL) are shown to be equally toxic and mutagenic in both g12 and V79 cells. UV and X-rays are also equally toxic to both cell lines. The data presented here suggests that g12 cells may be useful to study mammalian mutagenesis by agents which yield limited response at the hprt locus.

• OSTI ID: 5734023
• Journal Information: Environmental and Molecular Mutagenesis; (USA), Journal Name: Environmental and Molecular Mutagenesis; (USA) Vol. 16:1; ISSN 0893-6692; ISSN EMMUE
• Country of Publication: United States
• Language: English

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550200 -- Biochemistry
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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANIMAL CELLS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CLONING
CONNECTIVE TISSUE CELLS
DNA HYBRIDIZATION
DNA-CLONING
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ENZYMES
ESCHERICHIA COLI
EXTREME ULTRAVIOLET RADIATION
FIBROBLASTS
GENE RECOMBINATION
GENETIC EFFECTS
GENETIC ENGINEERING
GENETIC RADIATION EFFECTS
HYBRIDIZATION
HYDROGEN COMPOUNDS
IONIZING RADIATIONS
MICROORGANISMS
MUTAGENESIS
NITROSO COMPOUNDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
RADIATION EFFECTS
RADIATIONS
SOMATIC CELLS
TRANSFERASES
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
X RADIATION