HGPRT/sup -/ mutants of V79 cells that revert specifically by base pair substitution and frameshift mutations
Journal Article
·
· Environ. Mutagen.; (United States)
In order to determine the mutagenic specificity of mutagenic and carcinogenic agents in mammalian cells, a reversion system capable of distinguishing between frameshift mutations and various kinds of base pair substitutions would be useful. The authors report here a method for the isolation and characterization of HGPRT/sup -/ Chinese hamster V79 cell mutants that might form the basis for such a system. Two mutants of different specificity have been partially characterized. DEW-1, isolated following N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) treatment, is revertible by the base pair substitution mutagens MNNG and ethyl methanesulfonate (EMS), but not by frameshift mutagens. DSW-3, isolated following ICR-191 treatment, is specifically reverted by frameshift mutagens, but not by EMS or MNNG. With the further characterization of these and other mutants, it should be feasible to determine not only whether an agent is mutagenic in V79 cells, but also to determine the type(s) of mutations(s) it produces.
- Research Organization:
- New York Univ. School of Medicine, New York
- OSTI ID:
- 6378093
- Journal Information:
- Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 7:3; ISSN ENMUD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
CONNECTIVE TISSUE CELLS
EMS
ENZYMES
ESTERS
FIBROBLASTS
GLYCOSYL TRANSFERASES
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
LABELLED COMPOUNDS
MUTAGEN SCREENING
MUTAGENS
MUTANTS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PENTOSYL TRANSFERASES
REVERTANTS
SCREENING
SOMATIC CELLS
SULFONIC ACID ESTERS
TRANSFERASES
TRITIUM COMPOUNDS
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
CONNECTIVE TISSUE CELLS
EMS
ENZYMES
ESTERS
FIBROBLASTS
GLYCOSYL TRANSFERASES
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
LABELLED COMPOUNDS
MUTAGEN SCREENING
MUTAGENS
MUTANTS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PENTOSYL TRANSFERASES
REVERTANTS
SCREENING
SOMATIC CELLS
SULFONIC ACID ESTERS
TRANSFERASES
TRITIUM COMPOUNDS