Utilization of a quantitative mammalian cell mutation system, CHO/HGPRT, in experimental mutagenesis and genetic toxicology

Conference · Sat Jan 01 04:00:00 EST 1977

OSTI ID:7278027

Hsie, A. W.; Couch, D. B.; O'Neill, J. P.

Development of the CHO/HGPRT system is described and a host-mediated CHO/HGPRT assay is discussed. The following topics are discussed: evidence for the genetic origin of mutation induction in the CHO/HGPRT system; dose-response relationship for EMS-mediated mutation induction and cell lethality; apparent dosimetry of EMS-induced mutagenesis; structure-activity relationship of alkylating agents and ICR compounds; mutagenicity and cytotoxicity of congeners of two classes of nitrosi compounds; and preliminary validation of the CHO/HGPRT assay in predicting chemical carcinogenicity. (HLW)

• Research Organization: Oak Ridge National Lab., Tenn. (USA); Tennessee Univ., Oak Ridge (USA). Graduate School of Biomedical Sciences
• Sponsoring Organization: USDOE
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 7278027
• Report Number(s): CONF-770863-1
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALKYLATING AGENTS
ANIMAL CELLS
ANIMALS
ANTIMITOTIC DRUGS
BIOASSAY
BIOLOGICAL EFFECTS
CARCINOGENESIS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
EMS
ESTERS
EXPERIMENT PLANNING
GENETIC EFFECTS
MAMMALS
MUTAGENESIS
MUTAGENS
MUTATIONS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PATHOGENESIS
PLANNING
SULFONIC ACID ESTERS
VERTEBRATES