Strain construction strategies for chlorinated aromatic hydrocarbon-utilizers by multiple chemostat
Thesis/Dissertation
·
OSTI ID:7158781
A multiple chemostat method was used for the construction of a recombinant bacterium having the ability to degrade chlorinated aromatic hydrocarbons (CAH). This method permits natural genetic exchange between two parental strains and subsequent selection of a novel catabolic genotype in a defined system. Many recombinants which could utilize chlorobenzene (CB) as a sole source of carbon were constructed from several separate experiments in less than one month. Reproducibility is related to several important factors such as dilution rate, growth substrate, and surface attachment. A recombinant strain, Acinetobacter sp. CB15, which could grown on 3-chlorobiphenyl (PCB congener) with the release of inorganic chloride, was constructed by the application of the multiple chemostat method. This represents the first report of a pure culture able to grow on 3-chlorobiphenyl with the release of inorganic chloride. Two metabolites produced by Acinetobacter sp. CB15 grown on 3-chlorobiphenyl were identified as 3-chlorobenzoic acid and 3-chlorocatechol. An unidentified, dead-end, black metabolite with a UV absorption maximum at 255 nm was also produced. Recombinant strain CB15 could also cometabolize, 3,3{prime}-dichlorobiphenyl with the release of inorganic chloride when it was grown on biphenyl as the growth substrate.
- Research Organization:
- California Univ., Riverside, CA (USA)
- OSTI ID:
- 7158781
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
14 SOLAR ENERGY
140505* -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BIODEGRADATION
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
DECOMPOSITION
GENETIC ENGINEERING
GENOTYPE
GROWTH
HALOGENATED AROMATIC HYDROCARBONS
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
140505* -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BIODEGRADATION
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
DECOMPOSITION
GENETIC ENGINEERING
GENOTYPE
GROWTH
HALOGENATED AROMATIC HYDROCARBONS
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS