Construction of a 3-chlorobiphenyl-utilizing recombinant from an intergeneric mating. [Pseudomonas; Acinetobacter]

Journal Article · Sat Feb 01 04:00:00 EST 1992 · Applied and Environmental Microbiology; (United States)

OSTI ID:5560382

Adams, R H; Huang, C M; Higson, F K; Brenner, V; Focht, D D ^[1]

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1. Univ. of California, Riverside (United States)

Recombinant Pseudomonas sp. strain CB15, which grows on 3-chlorobiphenyl (3CB), was constructed from Pseudomonas sp. strain HF1, which grows on 3-chlorobenzoate, and from Acinetobacter sp. strain P6, which grows on biphenyl, by using a continuous amalgamated culture apparatus. DNA from strains CB15 and HF1 hybridized very strongly to each other, while hybridization between both parental strains, HF1 and P6, was negligible. However, DNA from the recombinant CB15 hybridized moderately to strongly with three specific fragments of parental strain P6. Strains HF1 and P6 did not grow on 3CB, but recombinant strain CB15 mineralized this compound and released inorganic chloride. When growing on 3CB, strain CB15 accumulated brown products, one of which was identified as 3-chloro-5-(2{prime}-hydroxy-3{prime}-chlorophenyl)-1,2-benzoquinone by mass spectrometry. At least three methods of inhibition from catecholic intermediates may account for slow growth on 3CB. In resting-cell assays, recombinant strain CB15 and strain P6 both metabolized 3CB faster than 3,3{prime}-dichlorobiphenyl. However, 3,3{prime}-dichlorobiphenyl could not be utilized as a growth substrate by strain CB15, nor did its presence have any effect on the rate of 3CB mineralization.

• OSTI ID: 5560382
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 58:2; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550500 -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
BACTERIA
BIODEGRADATION
BIOTECHNOLOGY
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
DECOMPOSITION
DNA
DNA HYBRIDIZATION
GENETIC ENGINEERING
GROWTH
HALOGENATED AROMATIC HYDROCARBONS
HYBRIDIZATION
INHIBITION
METABOLISM
METABOLITES
MICROORGANISMS
MINERALIZATION
NUCLEIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PSEUDOMONAS
RECOMBINANT DNA