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Continuous coculture degradation of selected polychlorinated biphenyl congeners by Acinetobacter spp. in an aerobic reactor system

Journal Article · · Environmental Science and Technology; (United States)
DOI:https://doi.org/10.1021/es00077a015· OSTI ID:5453413
;  [1]
  1. Univ. of California, Riverside (USA)

A coculture of two Acinetobacter spp. was applied to degrade polychlorinated biphenyls during a 42-day incubation study in a continuous aerobic fixed-bed reactor system, filled with polyurethane foam boards as support for bacterial biofilm development. The reactor was supplied with mineral medium containing 500 ppm sodium benzoate as a growth (primary) substrate, while the incoming airstream was saturated with biphenyl vapors to induce for PCB cometabolism in Acinetobacter sp. strain P6. The chlorobenzoates thus generated from 4,4{prime}-dichlorobiphenyl (4,4{prime}-DCBP), 3,4-dichlorobiphenyl (3,4-DCBP), and 3,3{prime},4,4{prime}-tetrachlorobiphenyl were further metabolized by Acinetobacter sp. strain 4-CB1. The chlorobenzoate metabolites, as well as ring-fission product ({lambda}{sub max} = 442 nm) from the PCB congeners, accounted for the degradation of 63% (2.8 mM) of the 4,4{prime}-DCBP, 100% (0.5 mM) of the 3,4-DCBP, and 32% (0.12 mM) of the 3,3{prime},4,4{prime}-TCBP, the biofilm responded with a concurrent higher release of chlorobenzoates and chloride through cosubstrate utilization.

OSTI ID:
5453413
Journal Information:
Environmental Science and Technology; (United States), Journal Name: Environmental Science and Technology; (United States) Vol. 24:7; ISSN ESTHA; ISSN 0013-936X
Country of Publication:
United States
Language:
English