Radioimmunoassay for intact gross mouse leukemia virus. [/sup 125/I tracer technique]

Journal Article · Sun Aug 01 04:00:00 EDT 1976 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.73.8.2847· OSTI ID:7156099

Yalow, R S ^[1]; Gross, L

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1. Solomon A. Berson Research Lab., Bronx, NY

A radioimmunoassay for intact Gross leukemia virus has been developed using /sup 125/I-labeled Gross virus grown in tissue culture and guinea pig antisera to Gross virus grown either in tissue culture or harvested from leukemic C3H(f) mice. Separation of bound from free labeled virus was effected using the double antibody method. The assay can detect fewer than 10/sup 8/ virus particles and has been used to measure the viral content of individual organs from inoculated leukemic C3H(f) mice and from AK mice with spontaneous leukemia. Organs from noninoculated healthy C3H(f) mice crossreacted poorly in the system, virus generally being detectable only in the thymus and spleen and low concentration. In some of the inoculated C3H(f) leukemic mice the viral content of as little as 0.5 ..mu..l of plasma is measurable. That this assay is for intact virus and not for soluble antigens of the viral envelope was proven by the observation that the immunoreactive material of plasma and extracts from thymus and liver of leukemic mice has a buoyant density in sucrose of 1.17--1.18 g/ml, corresponding to that of intact virus grown in tissue culture. With this sensitivity it may now be possible to quantitate viral concentrations in tissue and body fluids from the time of inoculation through the development of obvious pathology.

• OSTI ID: 7156099
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 73:8; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
HEMIC DISEASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKEMIA
MAMMALS
MICE
MICROORGANISMS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
PARASITES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RODENTS
TISSUE CULTURES
TRACER TECHNIQUES
VERTEBRATES
VIRUSES