Radiation effects on testes. X. Studies on inorganic pyrophosphatase and pyrophosphate following. gamma. irradition of rats

Journal Article · Fri Jul 01 04:00:00 EDT 1977 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574714· OSTI ID:7142664

Gupta, G S; Bawa, S R

Studies on inorganic pyrophosphatase and pyrophosphate of rat testes have been carried out after partial- and whole-body ..gamma.. irradiation of rats. Inorganic pyrophosphatase was diminished 18-20 hr after partial-body irradiation at 2000 R; the decline per milligram of protein continued up to 74 days with minimum values on days 10 and 24, respectively, after 2000- and 720-R exposures. When values were expressed per testis, however, the minima were observed on days 26 and 37 postirradiation, respectively, for the same exposures. The decreased levels of inorganic pyrophosphatase were accompanied by accumulation of pyrophosphate up to day 26 but with a maximum value on Day 10. The loss of inorganic pyrophosphatase is a more sensitive index for radiation injury of testes than is the loss of testis weight and loss of nucleic acids content. The loss of inorganic pyrophosphatase could be visualized at the nuclear and cytoplasmic levels after differential centrifugation. After whole-body irradiation at 720 R, the loss of inorganic pyrophosphatase was seen in liver, kidney, epididymis, and testes. The changes of inorganic pyrophosphatase and pyrophosphate were found to be associated with degenerative changes of testicular cells. It is concluded that further degeneration of testes is caused by accumulation of pyrophosphate which may be related to the reversal of several synthetic reactions in the germinal cells.

• Research Organization: Panjab Univ., Chandigarh, India
• OSTI ID: 7142664
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 71:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CHEMISTRY
ELECTROMAGNETIC RADIATION
ENZYMES
GAMMA RADIATION
GONADS
INJURIES
IONIZING RADIATIONS
MALE GENITALS
MAMMALS
ORGANS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PYROPHOSPHATES
RADIATION EFFECTS
RADIATION INJURIES
RADIATIONS
RATS
RODENTS
TESTES
VERTEBRATES