In vivo red cell destruction by anti-Lu6

Journal Article · Thu Mar 01 04:00:00 EST 1990 · Transfusion (Philadelphia); (USA)

DOI:https://doi.org/10.1046/j.1537-2995.1990.30390194349.x· OSTI ID:7137927

Issitt, P D; Valinsky, J E; Marsh, W L; DiNapoli, J; Gutgsell, N S ^[1]

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1. Duke Univ. Medical Center, Durham, NC (USA)

An example is presented of an IgG1, anti-Lu6, that reacted by indirect antiglobulin test and was capable of destroying antigen-positive red cells in vivo. Two methods for the measurement of red cell survival, {sup 51}Cr labeling and flow cytometry, gave the same result: 20 percent of the test dose of Lu:6 red cells was destroyed in the first hour after injection and 80 percent in the first 24 hours. The clinical relevance of the antibody was correctly predicted by an in vitro monocyte monolayer assay. The finding that this example of anti-Lu6 was clinically significant should not be taken to mean that all antibodies directed against high-incidence Lutheran and Lutheran system-related antigens will behave similarly. When such antibodies are encountered, in vivo and/or in vitro studies to assess their clinical significance are necessary before rare blood is used for transfusion.

• OSTI ID: 7137927
• Journal Information: Transfusion (Philadelphia); (USA), Journal Name: Transfusion (Philadelphia); (USA) Vol. 30:3; ISSN TRANA; ISSN 0041-1132
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD GROUPS
BODY FLUIDS
CELL FLOW SYSTEMS
CHROMIUM 51
CHROMIUM ISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ERYTHROCYTES
EVEN-ODD NUCLEI
GLOBULINS
IMMUNOASSAY
IMMUNOGLOBULINS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MAN
MATERIALS
NUCLEI
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOISOTOPES
SURVIVAL TIME
THERAPY
TRACER TECHNIQUES
TRANSFUSIONS
VERTEBRATES